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Detection of Ehrlichia canis in domestic cats in the central-western region of Brazil BJM
Braga,Ísis Assis; Santos,Luana Gabriela Ferreira dos; Ramos,Dirceu Guilherme de Souza; Melo,Andréia Lima Tomé; Mestre,Gustavo Leandro da Cruz; Aguiar,Daniel Moura de.
Ehrlichiosis is a worldwide distributed disease caused by different bacteria of the Ehrlichia genus that are transmitted by arthropod vectors. Its occurrence in dogs is considered endemic in several regions of Brazil. Regarding cats, however, few studies have been done and, consequently, there is not enough data available. In order to detect Ehrlichia spp. in cats from the central-western region of Brazil, blood and serum samples were collected from a regional population of 212 individuals originated from the cities of Cuiabá and Várzea Grande. These animals were tested by the Immunofluorescence Assay (IFA) and the Polymerase Chain Reaction (PCR) designed to amplify a 409 bp fragment of the dsb gene. The results obtained show that 88 (41.5%) cats were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ehrlichiosis; Feline; PCR; IFA; Ticks.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200036
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In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil BJM
Aguiar,Daniel M.; Hagiwara,Mitika K.; Labruna,Marcelo B..
An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ehrlichia canis; Dog; Molecular Characterization; Ehrlichiosis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014
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Spleen evaluation using contrast enhanced ultrasonography and Doppler in dogs with subclinical ehrlichiosis Arq. Bras. Med. Vet. Zootec.
Maronezi,M.C.; Feliciano,M.A.R.; Crivellenti,L.Z.; Borin-Crivellenti,S.; Silva,P.E.S.; Zampolo,C.; Pavan,L.; Gasser,B.; Simões,A.P.R.; Maciel,G.S.; Canola,J.C.; Vicente,W.R.R..
The aim of this study was to evaluate the splenic parenchyma of dogs with subclinical ehrlichiosis using Doppler and contrast-enhanced ultrasonography and provide reference values for this organ in affected animals. Seventeen dogs naturally infected with E. canis were selected for this study. Splenic parenchyma echotexture and echogenicity, size and borders were determined by ultrasound scan. The vascular indices of the splenic artery were determined by Doppler. SonoVue, at 0.1mL per animal, was used in microbubble contrast-enhanced ultrasonography to determine wash in, wash out and peak enhancement time in the splenic tissue. B-mode ultrasonography revealed splenomegaly with rounded borders, heterogeneous echotexture and mixed echogenicity. The vascular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Ehrlichiosis; Ultrasound.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601528
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